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Vesicular transport of Fe and interaction with other metal ions in polarized Caco2 Cell monolayers Biol. Res.
LINDER,MARIA C; MORIYA,MIZUE; WHON,ANNE; KASSA,AZEB; GILLEY,CYNTHIA.
Two aspects of the mechanisms by which iron is absorbed by the intestine were studied in the Caco2 cell model, using 59Fe(II)-ascorbate. Data showing the importance of vesicular processes and cycling of apotransferrin (apoTf) to uptake and overall transport of Caco2 cell monolayers (or basolateral 59Fe release) were obtained by comparing effects of: a) adding apoTf to the basal chamber; b) adding vesicular transport inhibitors; or c) cooling to 4ºC. These showed that apoTf may be involved in as much as half of Fe transfer across the basolateral membrane, and that vesicular processes may also play a role in non-apoTf-dependent Fe transport. Studies were initiated to examine potential interactions of other metal ions with Fe(II) via DMT1. Kinetic data showed...
Tipo: Journal article Palavras-chave: Intestinal iron absorption; Caco2 cells; Apotransferrin; Vesicular transport; Copper; Manganese.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100016
Registros recuperados: 61
Primeira ... 1234 ... Última
 

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